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目的:从全基因组角度探索羟基喜树碱对乳腺癌细胞MCF-7细胞信号转导相关基因的影响,寻找羟基喜树碱在乳腺癌治疗中潜在的新靶点.方法:HCPT诱导MCF-7细胞,MTT法检测HCPT对细胞增殖抑制.选择40mg/mL的HCPT作为诱导剂量组,并设置阴性对照,进行基因组芯片杂交实验.结果:HCPT对MCF-7细胞的增殖抑制作用均呈浓度依赖性,即随着HCPT浓度的增高,MCF-7的增殖受到明显抑制.HCPT诱导后,共获得了表达下调基因46个,表达上调基因9个,其中insulin-like growth factor binding protein1(IGFBP1)是表达下调(-4.01031)最大的信号转导基因,表达上调最大的信号转导相关基因是一广谱表达基因hemoglobin2(HBG2)(+3.804527),它在癌症发生中的作用未知.Q-PCR实验结果与芯片结果具有良好的一致性.结论:HCPT诱导乳腺癌细胞增殖抑制,可能涉及多个基因.
OBJECTIVE: To explore the effect of hydroxycamptothecin on the signal transduction related genes in breast cancer cell line MCF-7 from the genome-wide perspective and to find out potential new targets of hydroxycamptothecin in the treatment of breast cancer.Methods: HCPT induced MCF-7 Cells and MTT method were used to detect the inhibitory effect of HCPT on cell proliferation.HCPT of 40mg / mL was selected as the induction dose group and a negative control was set up to perform the experiment of genome chip hybridization.Results: HCPT inhibited the proliferation of MCF-7 cells in a concentration-dependent manner , That is, with the increase of HCPT concentration, the proliferation of MCF-7 was significantly inhibited.After HCPT induction, a total of 46 genes were down-regulated and 9 genes were up-regulated, of which insulin-like growth factor binding protein1 (IGFBP1) Down-regulation (-4.01031) The largest signal transduction gene, the most upregulated signal transduction related gene is a broad-spectrum gene hemoglobin2 (HBG2) (+ 3.804527), its role in the development of cancer is unknown.Q-PCR results And chip results have good consistency.Conclusion: HCPT can inhibit the proliferation of breast cancer cells, which may involve multiple genes.