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目的 以聚酯型儿茶素 (TS)为阳性对照 ,探讨其单体EGCG和GCG对人肠癌SW480细胞的生长抑制作用及其机制。方法 应用MTT法、软琼脂集落形成试验、Hoechst 3 3 2 5 8染色法和流式细胞术等方法探讨药物对细胞生长抑制作用。结果 单体EGCG和GCG与TS对SW 480细胞均呈剂量依赖性的抑制作用 ,其IC5 0值分别为 10 8 88、183 2 1、83 3 6μg·ml-1;以IC5 0浓度作用于SW 480细胞 2 4h后 ,EGCG、GCG、TS组的集落形成率显著低于对照组 (P <0 0 1) ;Hoechst 3 3 2 5 8染色观察到EGCG、GCG、TS组细胞出现明显的核浓缩或碎裂 ,其凋亡细胞比率显著高于对照组 (P <0 0 1) ;流式细胞术分析亦显示EGCG、GCG、TS组诱导的细胞凋亡率分别为对照组的 1 62、1 2 3、1 66倍。结论 与TS一致 ,EGCG、GCG对肠癌SW480细胞的生长有明显的抑制作用 ,其作用机制可能与诱导细胞凋亡有关。
Objective To investigate the inhibitory effect of EGCG and GCG on the growth of human colorectal cancer SW480 cells and its mechanism by using polyester-type catechin (TS) as a positive control. Methods MTT assay, soft agar colony formation assay, Hoechst 33258 staining and flow cytometry were used to investigate the inhibitory effects of drugs on cell growth. Results The monomeric EGCG and GCG inhibited the proliferation of SW480 cells in a dose-dependent manner with IC50 values of 10 8 88,183 2 and 18 3 36 μg · ml -1, respectively. 480 cells for 24 hours, the rates of colony formation in EGCG, GCG and TS groups were significantly lower than those in control group (P <0.01). Significant nuclear condensation of EGCG, GCG and TS groups was observed in Hoechst 3 3285 staining (P <0.01). The results of flow cytometry also showed that the apoptotic rates induced by EGCG, GCG and TS groups were significantly lower than that of the control group 2 3,1 66 times. CONCLUSIONS: Consistent with TS, EGCG and GCG significantly inhibit the growth of SW480 cells, which may be related to the induction of apoptosis.