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目的 :采用脉冲电场凝胶电泳技术 ,以释放出的有活性片段 (fraction of activity released,FAR)作为指标 ,评价DNA双链断裂 (DSB)将低估小量残余损伤 ,旨在避免由此而引起的误差。方法 :取在含 2 0 %小牛血清培养基中的人成纤维细胞(97%处于 G0 / G1 期 ) ,细胞内 DNA用 3H-胸
OBJECTIVE: To evaluate the use of fractional activity released (FAR) as a marker to assess DNA double-strand breaks (DSBs) that would underestimate the small residual damage using pulsed-field gel electrophoresis and avoidance of this The error. METHODS: Human fibroblasts (97% in G0 / G1 phase) were cultured in medium containing 20% fetal bovine serum. The intracellular DNA was digested with 3H-thymus