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从海南省临高县麻疯树种植基地的枯萎麻疯树树干木质部分离到一病原菌,该病菌从根部入侵,沿木质部导管向上蔓延,最终可导致整株植物萎蔫死亡.根据病害症状特点、病原菌形态特征、培养性状并结合rDNA-ITS区域的序列分析对病原微生物进行了鉴定.结果表明,麻疯树枯萎病的病原菌属半知菌亚门、丛梗孢目、镰孢属、尖孢镰刀菌(Fusarium oxysporum).采用平皿生长速率法测定了多菌灵可湿性粉剂、多菌灵·福美双可湿性粉剂、立枯净可湿性粉剂和代森锰锌可湿性粉剂四种药剂对病原菌的毒力,结果表明多菌灵效果最好,在200μg/mL时,对菌丝的抑制率为98.49%,多菌灵·福美双和立枯净次之,代森锰锌效果较差.
From the woody part of the Jatropha curcas tree in the Jatropha curcas planting base in Lincoli County, Hainan Province, it leaves a pathogen which invades from the roots and spreads upward along the xylem vessels, which eventually leads to wilting and death of the whole plant.According to the characteristics of the disease symptoms, Morphological characteristics, culture traits and sequence analysis of rDNA-ITS region were used to identify the pathogenic microorganisms.The results showed that the pathogenic microorganisms of Jatropha curcas were belong to the subdivision Amycolatopsis, Pteroceltis, Fusarium, (Fusarium oxysporum). The growth rate method was used to determine the carbendazim wettable powder, carbendazim, thiram wettable powder, wettable powder and mancozeb WP four agents on the pathogen The results showed that carbendazim had the best effect. The inhibition rate to mycelia was 98.49% at 200μg / mL, followed by carbendazim, thiram and clodronic, and the effect of mancozeb was worse.