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目的了解广州市某哨点医院门诊腹泻儿童蓝氏贾第鞭毛虫感染现状和分子流行病学特征。方法收集2012年6月～2013年5月广州市某哨点医院门诊腹泻儿童的人口资料学和粪便标本,采用巢氏聚合酶链式反应(nested-polymerase chain reaction,nested-PCR)方法检测粪便标本中的蓝氏贾第鞭毛虫。结果 348份粪便标本中蓝氏贾第鞭毛虫的检出率为4.60%(16/348),发病年龄以3岁以内为主,检出率在患者的性别差异无统计学意义(X2=1.29,P=0.256),检出高峰为7～10月。检出的16株蓝氏贾第鞭毛虫经测序分型,14个分离株为集聚型A,2个分离株为集聚型B,其中A-I亚型9株,A-II亚型5株,B-Ⅲ亚型2株。结论广州某哨点医院腹泻儿童存在蓝氏贾第鞭毛虫感染,检出高峰在7～10月,以3岁以内婴幼儿为主,主要流行集聚型A。
Objective To investigate the prevalence and molecular epidemiological characteristics of Giardia lamblia infection in outpatients with diarrhea at a sentinel hospital in Guangzhou. Methods The demographic data and stool specimens of children with diarrhea at a sentinel hospital in Guangzhou from June 2012 to May 2013 were collected. Nested-polymerase chain reaction (nested-PCR) Specimens of Giardia lamblia. Results The detection rate of Giardia lamblia in the 348 stool specimens was 4.60% (16/348). The age of onset was within 3 years of age. There was no significant difference in the detection rate among patients (X2 = 1.29 , P = 0.256). The peak was detected from July to October. 16 strains of Giardia lamblia were detected by sequencing, 14 strains were clustered A, 2 were aggregated B, of which 9 were AI, 5 were A-II, B -III subtype 2 strains. Conclusions Giardia lamblia infection was found in diarrhea children in a sentinel hospital in Guangzhou. The peak was detected in July to October. Infants and young children were mostly within 3 years old.