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目的:观察原矛头蝮(P.mucrosquamatus)蛇毒对小鼠脏器形态结构的影响,从组织学角度探讨该蛇毒的致病机理。方法:用原矛头蝮攻击小鼠,待小鼠死亡后,立即进行解剖,观察心脏和大血管内部血栓形成情况。取心脏、血管、周围神经、肾脏、肝脏、脾脏和肺,观察外形,并进行石蜡切片,H.E染色观察。用健康小鼠相同器官为对照,比较二者的差异。结果:被原矛头蝮攻击致死小鼠的心脏、肝脏和肺发生了显著收缩;心腔和血管腔被血栓堵塞;在心脏、肝脏和肾脏组织血管中观察到了血栓;脾脏中白细胞显著增多;肺泡收缩,细支气管严重收缩致管腔堵塞;股动脉血管内膜发生了脱落;在股神经中发生了脱髓鞘现象。结论:急性凝血和窒息是导致小鼠死亡的重要原因;原矛头蝮蛇毒兼具血循毒和神经毒的特征;原矛头蝮蛇毒可导致多种脏器内部结构发生改变。
OBJECTIVE: To observe the effect of P. mucrosquamatus snake venom on the morphology of mouse organs and to explore the pathogenesis of the snake venom from the histological point of view. Methods: Mice were challenged with the original spearheads. Immediately after the death of the mice, they were dissected to observe the internal thrombosis in the heart and large blood vessels. Take the heart, blood vessels, peripheral nerves, kidneys, liver, spleen and lungs, observe the shape, and paraffin sections, H.E staining. The same organs of healthy mice as a control, the difference between the two. RESULTS: Heart, liver and lungs of the lethal mouse attacked by Agkistrodon osprey were significantly contracted; the heart cavity and lumen were occluded by thrombus; thrombi were observed in the heart, liver and kidney tissue vessels; the spleen was markedly increased; alveoli Shrinkage, severe bronchial constriction to the lumen plug; femoral artery vascular intima occurred off; in the femoral nerve demyelination occurred. CONCLUSION: Acute coagulation and asphyxia are the important causes of death in mice. The original venomous snake venom has the characteristics of both blood and neurotoxicity. The original venomous snake venom can cause changes in the internal structure of various organs.