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目的探讨STAT3和p38在散发性大肠管状腺瘤癌变过程巾的可能作用。方法采用免疫组化方法检测107例散发性大肠管状腺瘤伴不同程度异型增生及癌变组织中STAT3和p38的蛋白表达水平,采用原位杂交方法检测STAT3 mRNA的表达。同时选取25例正常大肠黏膜作为对照。结果STAT3蛋白在正常大肠黏膜、大肠管状腺瘤伴上皮异型增生和癌变组中的阳性表达率分别为12.0%、59.0%和91.7%,STAT3 mRNA的阳性表达率分别为8.0%、51.8%和100.0%,p38蛋白的阳性表达率分别为8.0%、47.0%和91.7%。大肠管状腺瘤伴上皮异型增生组和癌变组STAT3蛋白、STAT3 mRNA和p38蛋白的阳性表达率均明显高于正常大肠黏膜组(P均<0.05),而癌变组均高于大肠管状腺瘤伴上皮异型增生组(P均<0.05)。大肠管状腺瘤伴上皮异型增生患者中,STAT3蛋白、STAT3 mRNA和p38蛋白的阳性表达率均随异型增生程度增高而增高(P<0.05)。STAT3与p38的表达呈正相关(r=0.515)。结论STAT3和p38均可能在散发性大肠管状腺瘤癌变过程中起一定作用。
Objective To investigate the possible role of STAT3 and p38 in the process of carcinogenesis of sporadic colorectal adenoma. Methods Immunohistochemistry was used to detect the protein expression of STAT3 and p38 in 107 cases of sporadic colorectal adenoma with different degrees of dysplasia and carcinogenesis. The expression of STAT3 mRNA was detected by in situ hybridization. At the same time select 25 cases of normal colorectal mucosa as a control. Results The positive rates of STAT3 protein in normal colorectal mucosa, tubular adenoma with epithelial dysplasia and cancerous lesions were 12.0%, 59.0% and 91.7%, respectively, and the positive rates of STAT3 mRNA were 8.0%, 51.8% and 100.0 %, the positive rate of p38 protein was 8.0%, 47.0% and 91.7% respectively. The positive rates of STAT3, STAT3 mRNA and p38 protein in colorectal adenoma with epithelial dysplasia and cancerous group were significantly higher than those in normal colorectal mucosa (all P <0.05), while those in cancerous group were higher than those in colorectal adenoma Epithelial dysplasia group (all P <0.05). The positive expression rate of STAT3, STAT3 and p38 in colorectal adenoma with epithelial dysplasia increased with the degree of dysplasia (P <0.05). STAT3 and p38 expression was positively correlated (r = 0.515). Conclusion Both STAT3 and p38 may play roles in the carcinogenesis of sporadic colorectal adenoma.