正常情况下多形核白细胞(PMN)仅是瞬时地粘附到血管内皮表面,在急性炎症时 PMN 沿血管壁移动,继之由内皮接触处穿过血管聚集到损伤部位。什么原因促使 PMN 粘附到血管特殊部位以及如何完成粘附尚不清楚。作者将内皮细胞和分离的 PMN 进行体外培养采用单层收集试验和扫描电镜观察、计数。发现PMN 和内皮细胞之间有相互作用,细胞表面电荷二价阳离子和趋化因子能影响这种作用,PMN 对内皮的粘附有明显特异性。扫描电镜计数有50%以上的牛 PMN 及36%的人 PMN 分别粘着在牛内皮细胞 Normally, polymorphonuclear leukocytes (PMN) adhere to the endothelium of the blood vessel only transiently, migrate along the vessel wall during acute inflammation, and then accumulate from the endothelium through the blood vessel to the site of injury. What is the reason for PMN adhesion to specific parts of the blood vessels and how to complete the adhesion is unclear. The authors cultured endothelial cells and isolated PMN in vitro using monolayer collection and scanning electron microscopy, counting. It was found that there was an interaction between PMN and endothelial cells. The cell surface charges of divalent cations and chemokines could affect this effect, and PMN had obvious specificity on the adhesion of endothelial cells. Scanning electron microscopy counted more than 50% of bovine PMN and 36% of human PMN adhering to bovine endothelial cells