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植物激素乙烯参与植物生长发育多个生理过程的调控。ACC氧化酶是植物乙烯合成途径中的关键合成酶。本研究依据其它物种ACC氧化酶序列信息设计简并引物,并结合RT-PCR和快速扩增c DNA末端技术从牧草百脉根中首次克隆到ACC氧化酶基因的全长c DNA序列,命名为LcACO1。LcACO1全长c DNA为1 012 bp,具有一个924 bp ORF可编码307个氨基酸残基。生物信息学分析表明,LcACO1是含有Fe2+依赖的加氧酶结构域的稳定亲水蛋白,没有信号肽和跨膜结构域,亚细胞定位于细胞质。与其它植物中的ACO蛋白进行同源性比对显示,LcACO1蛋白与鹰嘴豆中ACO蛋白的一致性高达90%。实时荧光定量PCR表明,LcACO1基因在百脉根不同组织器官中差异表达,根中表达量最高,推测其主要在根的生长发育调节方面发挥作用。
Regulation of Plant Hormone Ethylene in Multiple Physiological Process During Plant Growth and Development. ACC oxidase is a key synthase in the plant ethylene synthesis pathway. In this study, degenerate primers were designed based on the sequence information of ACC oxidase from other species. The full-length c DNA sequence of ACC oxidase gene was cloned from the roots of forage grass for the first time by RT-PCR and rapid amplification of c DNA end-technology, LcACO1. The full-length c DNA of LcACO1 is 1 012 bp, with a 924 bp ORF encoding 307 amino acid residues. Bioinformatics analysis showed that LcACO1 is a stable hydrophilic protein containing Fe2 + -dependent oxygenase domain, no signal peptide and transmembrane domain, and the subcellular localization in the cytoplasm. Homology alignments with ACO proteins from other plants showed that the LcACO1 protein confers up to 90% identity with the ACO protein in chickpea. Real-time fluorescence quantitative PCR showed that LcACO1 gene was expressed differently in different tissues and organs of Lotus corniculatus, and its expression level was the highest in root. It was speculated that LcACO1 gene mainly played a role in the regulation of root growth and development.