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目的 :通过对不同分化程度的子宫颈鳞状细胞癌细胞外间质 (ECM)胶原蛋白的表达进行检测 ,探讨胶原重构与癌细胞生长及浸润的关系。方法 :应用双重免疫荧光染色技术 ,对正常人及不同分化程度的子宫颈鳞状细胞癌病人子宫颈石蜡包埋组织的 、 和 型胶原表达进行检测 ,并应用共聚焦显微镜观察结果。结果 :正常子宫颈组织中 ,细胞外间质内可见结构致密的 、 型胶原的表达 , 型胶原蛋白呈线状环绕于血管、上皮下及一些间质细胞外。子宫颈鳞状细胞癌组织中 ,三型胶原均明显降解 ,表现为 、 型胶原结构稀疏 ,排列紊乱 ;癌细胞团周围基底膜内的 型胶原的线状荧光减弱、断裂甚至缺失 ,尤其以低分化鳞状细胞癌明显。结论 :子宫颈鳞状细胞癌时 , 、 、 型胶原降解 ,ECM重构 ,其程度与癌细胞分化程度呈负相关。
Objective: To explore the relationship between collagen remodeling and cancer cell growth and infiltration by examining the expression of extracellular matrix (ECM) collagen in cervical squamous cell carcinoma with different degree of differentiation. Methods: The double-immunofluorescence staining technique was used to detect the expression of type I and type IV collagen in cervical squamous cell carcinoma tissues of normal and different degree of cervical squamous cell carcinoma patients. The results were observed by confocal microscopy. Results: In normal cervical tissue, the structure of dense and collagen was observed in the extracellular matrix. Collagen type collagen was surrounded by blood vessels, subepithelial and extra stromal cells. Cervical squamous cell carcinoma tissue, type three collagen were significantly degraded, manifested as sparse collagen arrangement, disordered arrangement; cancer cell mass around the basement membrane type collagen collagen weakening, rupture or even missing, especially low Differentiated squamous cell carcinoma is obvious. Conclusion: Cervical squamous cell carcinoma, collagen degradation, ECM remodeling, the degree of cancer cells and the degree of differentiation was negatively correlated.