目的 :研究肿瘤坏死因子α诱导蛋白3(tumor necrosis factor alpha induced protein 3,TNFAIP3)、锰超氧化物歧化酶(Manganese superoxide dismutase,Mn-SOD)m RNA在放射治疗后鼻咽癌中的表达,探讨其在鼻咽癌放射治疗中的意义。方法 :运用原位杂交方法检测放射治疗后鼻咽癌中TNFAIP3及Mn-SOD m RNA的表达。结果:TNFAIP3 m RNA中度及强阳性表达率在放疗敏感鼻咽癌中为15.00%,在放疗抗拒鼻咽癌中为45.00%;Mn-SOD m RNA中度及强阳性表达率在放疗敏感鼻咽癌中为55.00%,在放疗抗拒鼻咽癌中为40.00%,差异均有统计学意义(P均<0.05)。在放疗抗拒鼻咽癌中,TNFAIP3 m RNA中度及强阳性表达率与TNM分期呈正相关,Ⅲ~Ⅳ期表达率为48.28%,Ⅰ~Ⅱ期表达率为36.36%,Mn-SOD m RNA中度及强阳性表达率与T分期呈正相关,T3~T4期中表达率为63.16%,T1~T2期中表达率为19.05%;TNFAIP3 m RNA中度及强阳性表达率在有远处转移的放疗抗拒鼻咽癌中为81.81%,在无远处转移的放疗抗拒鼻咽癌中为31.03%,Mn-SOD m RNA中度及强阳性表达率在有远处转移的放疗抗拒鼻咽癌中为81.82%,在无远处转移的放疗抗拒鼻咽癌中为24.14%,差异均有统计学意义(P均<0.05)。结论:TNFAIP3参与了放疗抗拒鼻咽癌的发生、发展;Mn-SOD能增加鼻咽癌的放射敏感性,增强放疗鼻咽癌的抗氧化作用。 Objective: To investigate the expression of tumor necrosis factor alpha induced protein 3 (TNFAIP3) and manganese superoxide dismutase (Mn-SOD) m RNA in nasopharyngeal carcinoma after radiotherapy, To explore its significance in radiation therapy of nasopharyngeal carcinoma. Methods: The expression of TNFAIP3 and Mn-SOD mRNA in nasopharyngeal carcinoma after radiotherapy was detected by in situ hybridization. Results: The moderate and strong positive expression rate of TNFAIP3 m RNA was 15.00% in radiotherapy-sensitive nasopharyngeal carcinoma and 45.00% in radiotherapy-resistant nasopharyngeal carcinoma. The moderate and strong positive expression rates of Mn-SOD m RNA in radiosensitive nasal mucosa 55.00% in pharyngeal carcinoma and 40.00% in radiotherapy-resistant nasopharyngeal carcinoma, the differences were statistically significant (all P <0.05). In radiotherapy-resistant nasopharyngeal carcinoma, the moderate and strong positive expression rates of TNFAIP3 m RNA were positively correlated with the TNM stage, the expression rates of stage Ⅲ-Ⅳ were 48.28%, the expression rates of stage Ⅰ-Ⅱ were 36.36% The positive rate of TFA was positively correlated with T stage, the positive rate was 63.16% in T3 ~ T4 stage and 19.05% in T1 ~ T2 stage. The moderate and strong positive expression rates of TNFAIP3 m RNA in radiotherapy with distant metastasis 81.81% in nasopharyngeal carcinoma and 31.03% in radiotherapy-resistant nasopharyngeal carcinoma without distant metastasis. The moderate and strong positive expression rate of Mn-SOD m RNA in nasopharyngeal carcinoma with distant metastasis was 81.82 %, 24.14% in radiotherapy-resistant nasopharyngeal carcinoma without distant metastasis, the differences were statistically significant (all P <0.05). Conclusion: TNFAIP3 participates in the radiotherapy to prevent the occurrence and development of nasopharyngeal carcinoma. Mn-SOD can increase the radiosensitivity of nasopharyngeal carcinoma and enhance the anti-oxidation of nasopharyngeal carcinoma.