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以唐菖蒲‘Advanced Red’品种的籽球为试验材料,将籽球切片放在MS+6.0mg·L-1 TDZ培养基上暗培养21d,诱导胚性愈伤组织;将胚性愈伤组织转入MS+1mg·L-12,4-D培养基中暗培养28d诱导体细胞胚。使用立体显微镜和石蜡切片技术对体胚形态与细胞组织进行观察。结果表明:体胚诱导14d后,开始出现一些胚性细胞,之后形成多个细胞组成的原胚(pre-embryoni ccell complexes),原胚进而发育形成球形、盾形,心形、鱼雷形胚和子叶期胚。将成熟的体胚转入MS培养基后长出不定根和不定芽,形成完整的植株。唐菖蒲体胚的起源有外起源和内起源两种方式。在初生体胚发育过程中还伴有次生胚的形成。
The seeds of Gladiolus ’Advanced Red’ were used as experimental materials, and the seed-bulb slices were placed in MS medium supplemented with 6.0mg · L-1 TDZ for 21 days to induce embryogenic callus. The embryogenic callus Transferred into MS + 1mg · L-12,4-D medium for 28 days to induce somatic embryos. The morphology of the somatic embryos and the tissue of the cells were observed using stereomicroscopy and paraffin sections. The results showed that some embryogenic cells began to appear after 14 days of somatic embryogenesis, and then many pre-embryonic ccell complexes were formed. The embryos developed spherical, conical, heart-shaped and torpedo-shaped embryos Cotyledons embryo. The mature somatic embryos transferred MS medium after adventitious roots and adventitious buds grow to form a complete plant. Gladiolus somatic embryo origins have two kinds of origin and origin. Secondary embryo formation is also accompanied by primary somatic embryogenesis.