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【目的】精细定位和克隆陆地棉光敏雄性不育基因。【方法】从陆地棉中040029的航天诱变后代中选育出新型光敏雄性不育系中9106,以中9106与11个陆地棉材料配制杂交组合,初步分析了中9106的杂种优势。以中9106为母本与陆地棉乐土603构建遗传分离群体F1、F2,分析不育性状的遗传特征。利用集团分离分析法筛选简单序列重复(Simple sequence repeat,SSR),并在包含186个单株的中9106×乐土603 F2群体中用上述SSR初步定位不育基因。【结果】中9106为母本×陆地棉乐土603的F1单株育性均表现正常,而F2群体中的可育单株数∶不育单株数符合3∶1的分离比,推测中9106的不育性状受1对隐性核基因控制。利用集团分离分析法从16 544对SSR中筛选到18对与该不育基因连锁的标记。利用中9106×乐土603的F2群体和18对SSR标记,将不育基因定位于D12染色体,位于标记NAU3442与CGR6339之间,遗传距离均为0.2c M,将该不育基因命名为ys-1。【结论】本研究有助于ys-1的精细定位及其克隆。
【Aim】 Finely orient and clone Upland cotton genic male sterile gene. 【Method】 A new type of photosynthetic male sterile line 9106 was bred from space mutagenesis progeny of upland cotton 040029. The hybrid combinations of 9106 and 11 cotton cultivars were preliminarily analyzed, and the heterosis of medium 9106 was analyzed. The genetic segregation populations F1 and F2 were constructed with Zhong 9106 as female parent and upland 603 as the soil, and the genetic characteristics of infertility traits were analyzed. The simple sequence repeat (SSR) was screened by group segregation analysis and the SSRs were initially mapped in F2 population of 9106 × Land 603 with 186 individuals. 【Result】 The results showed that the F1 single plant fertility in 9106 as female parent × P. australis 603 was normal, while the number of fertile single plants in F2 population: the number of male sterile plants was in accordance with the segregation ratio of 3:1. Fertility traits are controlled by a pair of recessive nuclear genes. 18 of 16 544 pairs of SSRs were screened by group segregation method for the markers linked to this sterile gene. The F2 population and 18 pairs of SSR markers in 9106 × Lotto 603 were used to locate the sterile gene on chromosome D12 and located between markers NAU3442 and CGR6339 with a genetic distance of 0.2cM. The sterile gene was named as ys-1 . 【Conclusion】 This study is helpful for the fine mapping and cloning of ys-1.