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硝态氮除作为主要氮源外,还作为一种潜在的信号物质,在植物生长发育过程中起着重要作用,其作用方式的直接性或间接性近年来成为了研究热点。利用双向电泳(2-DE)技术,对不同形态氮素(NO3- 与NH4+)培养的水稻叶片蛋白表达谱进行了比较分析,结果在硝态氮和铵态氮培养的叶片中分别分辨出26 个和6 个增量表达蛋白。质谱分析结合数据库检索鉴定出11 个蛋白,其中7 个硝态氮上调的蛋白为:光系统II 放氧复合蛋白1(N1)、抗性相关蛋白MLA13(N2)、光系统II23kD多肽(N3)、翻译激活因子(N5)、光系统II 放氧复合蛋白2 前体(N8)、未知蛋白(N17)和泛素载体蛋白(N18);4个铵态氮上调蛋白为:ATP合酶β亚基(A1)、转氨酶(A3)和两个功能未知的蛋白(A5,A6)。上述研究结果有助于了解水稻适应不同氮素营养时的生物化学基础及可能的生物学意义,同时也为深入阐明水稻响应NO3-与NH4+ 信号的反应提供了蛋白水平的证据。
In addition to being the main nitrogen source, nitrate nitrogen also plays an important role as a potential signal substance in plant growth and development, and the directness or indirectness of its mode of action has become a research hotspot in recent years. Two-dimensional electrophoresis (2-DE) technique was used to compare the leaf protein expression patterns of rice cultivated in different forms of nitrogen (NO3- and NH4 +). The results showed that 26 One and six incremental expression proteins. Eleven proteins were identified by mass spectrometry and database search. Among them, seven of the up-regulated proteins of nitrate nitrogen were: photoperiod II oxygen-binding protein 1 (N1), resistance-related protein MLA13 (N2), photosystem II23kD polypeptide (N3) , N5, N8, N17 and U18. The four ammonium nitrogen up-regulated proteins were: ATP synthase β subunit (A1), aminotransferase (A3) and two proteins of unknown function (A5, A6). The above results are helpful to understand the biochemical basis and possible biological significance of rice in adapting to different nitrogen nutrition, and also provide evidence for further clarifying the response of rice to the reaction of NO3- and NH4 + signal.