美国加州大学旧金山分校的Bell等人继解决大鼠胰岛素基因核苷酸顺序后,于今年三月宣布他们又解决了人胰岛素基因的核苷酸顺序。他们先从人胰岛素瘤提取胰岛素的信使核糖核酸(mRNA),经反向转录得到与此胰岛素mRNA互补的脱氧核糖核酸(cDNA)。为了得到更多量的cDNA,他们将其组入细菌质体(plasmid)DNA中,利用质体的 Bell et al. Of the University of California, San Francisco, announced in March this year that they have solved the nucleotide sequence of the human insulin gene again after resolving the nucleotide sequence of the rat insulin gene. They first extracted the insulin messenger RNA from human insulinomas and reverse transcribed it to get the DNA that is complementary to the insulin mRNA. In order to obtain more amounts of cDNA, they are grouped into bacterial plasmid DNA,