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观察染色体应用甘蔗根尖压片方法较之用花粉母细胞压片方法有取材方便的优点(前者已在《甘蔗分刊》1980年第5期介绍),此法无需特殊和贵重药物进行处理,只要采样时间恰当,操作方法正确,一般是可以获得理想的分裂相细胞并观察到染色体的。但是,它仍有某方面的局限性,如利用甘蔗花药培养诱导出来的幼小植株或经辐射诱变甘蔗愈伤组织诱导出来的少量材料来检查细胞染色体,则会因根系损伤而影响幼小植株的成活率,而且每个单株最多只能剪取1～2条根尖来制片,因此,往
The observation of chromosome application sugarcane tip tablet method compared with the pollen mother cell compression method has the advantages of drawing convenience (the former has been in the “sugarcane” fifth issue 1980 introduction), this method without special and expensive drugs for processing, As long as the sampling time is right and the method of operation is correct, it is generally possible to obtain the ideal split phase cells and observe the chromosomes. However, there are still some limitations. For example, young plants induced by sugarcane anther culture or small amounts of materials induced by radiation-induced sugarcane callus are used to examine the chromosomes of the cells, which may affect the young plant due to root damage Survival rate, and each plant can only cut 1 to 2 root tips to produce, therefore, to