Objective: To fabricate polymeric nanocomposites with excellent photoluminescence, magnetic properties, and stability in aqueous solutions, in order to improve speciifcity and sensitivity of cellular imaging under a magnetic ifeld.
Methods:Fluoridated Ln3+-doped HAP (Ln3+-HAP) NPs and iron oxides (IOs) can be encapsulated with biocompatible polymers via a modiifed solvent exaction/evaporation technique to prepare polymeric nanocomposites with lfuoridated Ln3+-HAP/iron oxide. The nanocomposites were characterized for surface morphology, fluorescence spectra, magnetic properties andin vitro cytotoxicity. Magnetic targeted cellular imaging of such nanocomposites was also evaluated with confocal laser scanning microscope using A549 cells with or without magnetic ifeld.
Results: hTe fabricated nanocomposites showed good stability and excellent luminescent properties, as well as lowin vitro cytotoxicity, indicating that the nanocomposites are suitable for biological applications. Nanocomposites under magnetic ifeld achieved much higher cellular uptake via an energy-dependent pathway than those without magnetic ifeld.
Conclusion: hTe nanocomposites fabricated in this study will be a promising tool for magnetic targeted cellular imaging with improved speciifcity and enhanced selection.