论文部分内容阅读
目的:检测48例T细胞非霍奇金淋巴瘤(T-NHL)6q14-26上12个高度多态性微卫星标记物的杂合性缺失(LOH)情况,确定与T-NHL相关的6q上的最小缺失区(RMDs),为寻找与T-NHL发病相关的抑癌基因提供有意义的信息。方法:选取6q14-26区段上12个多态性微卫星标记,行聚合酶链式反应-单链长度多态性分析,检测LOH,确定发生LOH的最小重叠区段,并分析6qLOH与肿瘤样本临床病理特征的关系。结果:48例肿瘤样本中8例(17%)至少有一个位点出现LOH,12个标记物LOH的频率从8%到50%不等,RMD位于6q21-22,另发现一个发生LOH频率较高的部位位于6q25。6qLOH在低度恶性与高度恶性T-NHL患者之间差异有显著性(P=0.044)。结论:6qLOH与T-NHL的恶性程度有统计学相关性。6q21-22和6q25区段可能存在一个或多个候选肿瘤抑制基因,这些抑癌基因的失活可能在T-NHL的发病机制中发挥重要作用。
OBJECTIVE: To detect the loss of heterozygosity (LOH) of 12 highly polymorphic microsatellite markers on 48 T-cell non-Hodgkin’s lymphoma (T-NHL) 6q14-26, and to identify the 6q (RMDs) provide meaningful information for finding tumor suppressor genes related to the pathogenesis of T-NHL. Methods: Twelve polymorphic microsatellite markers in 6q14-26 region were selected for polymerase chain reaction-single-strand length polymorphism analysis to detect LOH, to determine the minimum overlap region of LOH, and to analyze the relationship between 6qLOH and tumor The relationship between the clinicopathological features of samples. Results: LOH was found in at least one of the 8 cases (17%) of 48 tumor samples. The frequency of LOH in 12 markers ranged from 8% to 50%, RMD was located in 6q21-22. Another frequency of LOH was found The high site located at 6q25.6qLOH was significantly different between patients with low-grade and high-grade T-NHL (P = 0.044). Conclusion: There is a statistically significant correlation between the malignant degree of 6qLOH and T-NHL. The 6q21-22 and 6q25 segments may contain one or more candidate tumor suppressor genes. Inactivation of these tumor suppressor genes may play an important role in the pathogenesis of T-NHL.