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目的 观察人食道癌细胞株RNA致敏的树突状细胞 (DC)所诱导的特异性抗肿瘤免疫效应 ,探讨肿瘤细胞RNA直接致敏DC进行食道癌生物治疗的可能性。方法 食道癌细胞株TTn体外培养 ,提取RNA ;正常人外周血 ,进行体外DC的培养扩增 ;食道癌RNA直接致敏DC ,MTT法检测食道癌RNA活化DC所诱生的淋巴细胞对食道癌TTn、视网膜母细胞瘤SoRb -70的体外杀伤效应。结果 正常人外周血来源的DC ,经食道癌TTn细胞株RNA直接致敏后 ,成功诱导特异性抗肿瘤免疫反应 ,TTnRNA组在CTL在靶效比为 2 0 ;1时对TTn、SoRb -70的杀伤率分别在 84 5 4%、1 5 3 %,而对照组对这两种细胞的杀伤率分别为 1 3 4%和 1 70 %。结论 肿瘤RNA致敏DC可以诱导特异性抗肿瘤免疫 ,是一种有前景的食道癌治疗手段 ,有进一步研究的价值
Objective To observe the specific anti-tumor immune effect induced by RNA-sensitized dendritic cells (DCs) of human esophageal cancer cell lines, and to explore the possibility of biological treatment of esophageal cancer by directly sensitizing tumor cells with DCs. Methods Human esophageal cancer cell line TTn was cultured in vitro and RNA was extracted. Peripheral blood of normal people were cultured and expanded in vitro. DCs of esophageal cancer were directly sensitized to DC. MTT was used to detect the effect of lymphocytes induced by activated RNA of esophageal cancer on esophageal cancer TTn, retinoblastoma SoRb -70 in vitro cytotoxicity. Results DCs from normal human peripheral blood were directly sensitized by RNA of TTn cell line of esophageal cancer, and specific antitumor immune response was induced successfully. TTnRNA group had a significant effect on TTn, SoRb-70 Killing rates were 84 5 4% and 15 3% respectively, while the killing rates of the control group to these two kinds of cells were respectively 134% and 170%. Conclusions Tumor RNA-sensitized DC can induce specific anti-tumor immunity, which is a promising therapeutic approach for esophageal cancer and has further research value