用主成分分析探索不同中药组分配伍干预脑缺血的基因表达模式

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目的比较清开灵主要组分黄芩苷、栀子苷及其配伍干预脑缺血损伤的基因表达模式。方法12只4周龄小鼠随机分为黄芩苷组(BA组)、栀子苷组(JA组)、栀子苷+黄芩苷组(BJ组)、尼莫地平组(NM组),建立全脑缺血再灌注小鼠模型后,各组分别给予尾静脉注射黄芩苷、栀子苷、栀子苷+黄芩苷以及尼莫地平。抽提小鼠脑组织的总RNA,制备小鼠全基因组表达谱,对其进行基因本体论功能分类,构建相关系数矩阵,对差异表达的细胞黏附相关基因进行主成分分析。结果获取16463个小鼠脑测全基因组表达谱,筛选出32个差异表达的细胞黏附相关基因。主成分分析结果显示,4组的前2个主成分的累积贡献度为100%,BJ组总得分大于单一组分组,NM组对细胞黏附相关基因干预的总得分高于BA和JA组。各次检测的主成分分布显示,NM组和3个中药组均距离较远,BJ组和BA组的距离均近于JA组。结论黄芩苷、栀子苷及其配伍均能有效干预细胞黏附相关基因,配伍后的干预对黏附相关功能基因的影响大于单一组分组,其基因表达模式和黄芩苷相近。 Objective To compare the gene expression patterns of baicalin, geniposide and their compatibility in the treatment of cerebral ischemic injury. Methods Twelve mice of 4 weeks old were randomly divided into baicalin group (BA group), geniposide group (JA group), geniposide + baicalin group (BJ group) and nimodipine group (NM group) After the model of global cerebral ischemia-reperfusion in mice, each group were given intravenous injection of baicalin, geniposide, geniposide + baicalin and nimodipine. The total RNA was extracted from the brain tissue of mice and the whole genome expression profile of mice was prepared. The gene ontology function classification was carried out to construct the correlation coefficient matrix, and the principal component analysis of differentially expressed cell adhesion related genes was carried out. Results Totally 16463 mice were enrolled and the whole genome expression profile was obtained. Thirty-two differentially expressed cell adhesion related genes were screened out. The principal component analysis showed that the cumulative contribution of the first two principal components in the four groups was 100%, the total score of the BJ group was larger than that of the single group, and the total score of the NM group on the cell adhesion-related gene intervention was higher than that of the BA and JA groups. The principal component distribution of each test showed that NM group and three Chinese medicine group were far away from each other, and the distance between BJ group and BA group was close to that of JA group. Conclusions Baicalin, Geniposide and their compatibility can effectively interfere with cell adhesion related genes. The effect of compatibility on the adhesion-related functional genes is greater than that of the single group. The gene expression pattern is similar to that of baicalin.
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