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目的:研究蛞蝓多糖体外抗乙型肝炎病毒的作用。方法:以不同浓度的蜗牛多糖和拉米夫定与HepG2.2.15细胞混合培养,通过MTT法检测药物的细胞毒性,9d后用ELISA法检测细胞培养液中的HBsAg和HBeAg分泌水平,用实时荧光定量PCR技术检测培养液中的HBV-DNA含量。结果:蛞蝓多糖在1mg·mL-1浓度以下对细胞无毒性。蛞蝓多糖在所稀释的各浓度下,对HBsAg、HBeAg均有抑制作用,最大抑制率分别为56.2%和58.1%;HBsAg、HBeAg的治疗指数分别大于19.98%、22.78%。蛞蝓多糖可抑制HBV-DNA的复制(P<0.05)。结论:蛞蝓多糖在体外具有显著抑制HBV的作用。
Objective: To study the anti-hepatitis B virus effect of Lycium barbarum in vitro. METHODS: Different concentrations of snail polysaccharide and lamivudine were mixed with HepG2.2.15 cells. The cytotoxicity of the drug was tested by MTT assay. After 9 days, the HBsAg and HBeAg secretion levels in the cell culture medium were detected by ELISA method. Quantitative PCR was used to detect HBV-DNA content in the culture fluid. Results: Lycium barbarum polysaccharide was not toxic to cells at a concentration of 1 mg·mL-1. The lycium barbarum polysaccharides had inhibitory effects on HBsAg and HBeAg at the diluted concentrations. The maximum inhibition rates were 56.2% and 58.1%, respectively. The therapeutic indexes of HBsAg and HBeAg were greater than 19.98% and 22.78%, respectively. LPS could inhibit the replication of HBV-DNA (P<0.05). Conclusion: LBP has a significant inhibitory effect on HBV in vitro.