应用流式细胞术，从ＨＬＡＡ２阳性人黑色素瘤细胞系（６２４Ｍｅｌ）中选择培养，鉴定出一株丢失ＨＬＡ—Ａ２分子的黑色素瘤细胞（６２４—ｎＭｅｌ），它具有抵抗ＨＬＡＡ２限制的、黑色素瘤抗原肽特异性ＴＩＬ杀伤作用。经ｒＩＦＮγ诱导，能提高６２４－ＭｅｌＨＬＡ－Ａ２分子表达，而不能增强６２４ｎＭｅｌＨＬＡ－Ａ２分子表达和其对ＴＩＬ的敏感性。结果提示，ＴＩＬ对瘤细胞抗原的识别依赖于ＨＬＡⅠ类分子，而瘤细胞ＨＬＡⅠ类分子的缺失可能是肿瘤逃逸机体免疫监视的一个重要因素。 Application of flow cytometry, from HLA A2 positive human melanoma cell line (624 Mil) culture, identified a loss of HLA-A2 molecules in melanoma cells (624-nMel), which has resistance to HLA A2 Restricted, melanoma antigen peptide specific TIL killing effect. Induced by rIFN  γ, can improve 624-MelHLA-A2 molecule expression, but can not enhance the 624  nMelHLA-A2 molecule expression and its sensitivity to TIL. The results suggest that the recognition of tumor cell antigen by TIL depends on HLA class I molecules, and the deletion of HLA class I molecules in tumor cells may be an important factor in the immune surveillance of tumor escape.