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目的用DNA混合池与微卫星遗传标记寻找2型糖尿病的关联遗传位点,定位致病基因。方法在2号染色体上间隔10厘摩(cmol/L)遗传距离选择了30个微卫星遗传标记,分别对284例有家族史的2型糖尿病患者(T2DM组)和1000名正常对照者(NC组)各自组成的DNA混合样本进行扫描,比较T2DM组与NC组每个等位基因频率的差异。结果在D2S286与D2S335位点T2DM组和NC组的等位基因频率存在统计学差异(P≤0.01)。结论山东省汉族人群家族史阳性的2型糖尿病患者群体在2号染色体D2S286和D2S335位点存在关联,为筛查易感基因提供了线索。
Objective To search for the associated genetic loci of type 2 diabetes with DNA hybridization pool and microsatellite markers to locate the pathogenic genes. Methods Thirty microsatellite loci were selected on chromosome 2 with a genetic distance of 10 cmol / L, and 284 family members with type 2 diabetes (T2DM group) and 1000 normal controls (NC group) Group) were screened, and the differences of allele frequencies between T2DM group and NC group were compared. Results There was a significant difference in allele frequency between T2DM group and NC group at D2S286 and D2S335 loci (P≤0.01). Conclusion The population of type 2 diabetic patients with positive family history of Han nationality in Shandong Province is associated with the D2S286 and D2S335 sites on chromosome 2, providing a clue for screening susceptibility genes.