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Sera from patients with different courses of chronic renal failure and healthy persons were separated by gel permeation chromatography,and the concentration of middle molecular fraction B were calculated.The concentration of fractions B was increasing with the aggravating of renal failure degree,which indicated that the concentration of fraction B was related to patients′ renal failure.Fractions B from uremic urine,uremic sera or normal urine were resolved into 10 to 17 sub-fractions by ion exchange chromatography.Sub-fractions from normal urine and from uremic sera were assayed for their inhibition for the activity of Na,K-ATPase.The results showed that the Na,K-ATPase activities of the samples with B-8 and B-9 were much lower than that of blank.It is indicated that B-8 and B-9 contained some compounds which could inhibit the activity of Na,K-ATPase.
Sera from patients with different courses of chronic renal failure and healthy individuals were separated by gel permeation chromatography, and the concentration of middle molecular fraction B were calculated with the aggravating of renal failure degree, which indicated that the concentration of fraction B was related to patients’ renal failure. Fraction B from uremic urine, uremic sera or normal urine were resolved into 10 to 17 sub-fractions by ion exchange chromatography. Sub-fractions from normal urine and from uremic sera were assayed for their inhibition for the activity of Na, K-ATPase. The results showed that the Na, K-ATPase activities of the samples with B-8 and B-9 were much lower than that of blank. It is indicated that B-8 and B-9 contained some compounds which could inhibit the activity of Na, K-ATPase.