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目的 :探讨端粒酶反义寡核苷酸对二甲肼诱导大鼠大肠癌的预防作用。方法 :用 1,2 二甲肼 ( 1,2 DMH)诱导SD大鼠肠癌发生的模型 ,观察端粒酶反义寡核苷酸对端粒酶活性及二甲肼诱癌率的影响。结果 :本研究使用二甲肼成功地诱导了大鼠大肠癌发生模型 ,阴性对照组未见肿瘤发生 ,阳性诱癌组诱癌率为 5 7 6 9% ,反义寡核苷酸保护组诱癌率为 2 6 92 % ,明显低于阳性诱癌组 (P <0 0 1) ;同时反义寡核苷酸保护组端粒酶活性低于阳性诱癌组 (P <0 0 5 )。结论 :端粒酶反义寡核苷酸在体内能抑制端粒酶活性 ,对DMH诱导的肠癌有预防作用 ,可望成为预防和治疗大肠癌的有效药物
Objective: To explore the preventive effect of telomerase antisense oligonucleotides on dimethyl sulphonium-induced colorectal cancer in rats. METHODS: A model of colon cancer in SD rats was induced with 1,2 dimethyl hydrazine (1,2 DMH). The effects of telomerase antisense oligonucleotides on telomerase activity and dimethyl hydrazine induced cancer rate were observed. RESULTS: In this study, dimethyl hydrazine was used to successfully induce the rat colorectal cancer model. No tumor occurred in the negative control group. The tumor-inducing rate was 576.9% in the positive tumor-inducing group. The antisense oligodeoxynucleotide protection group induced The cancer rate was 269.2%, which was significantly lower than that of the positive tumor-inducing group (P <0 01). The antisense oligodeoxynucleotide protection group had lower telomerase activity than the positive tumor-inducing group (P <0 05). Conclusion: Telomerase antisense oligonucleotides can inhibit telomerase activity in vivo and have a preventive effect on DMH-induced colon cancer. It is expected to be an effective drug for preventing and treating colorectal cancer.