脱落细胞学检查是临床病理检查的常用方法,依靠细胞学检查判断其的良恶性,能为临床早期发现、早期治疗恶性肿瘤提供可靠的诊断依据。传统的细胞学检查依靠光镜观察细胞核形态,有时由于背景成分的干扰,或者形态不典型,容易受到病理医师的主观因素影响。我们将DNA倍体分析与免疫组化染色运用到疑难病例的诊断中,希望有助于疑难细胞学的诊断。1材料与方法1.1材料选取成都军区总医院2013-09—2014-12间疑难 Exfoliative cytology is a common method of clinical and pathological examination, relying on cytology to determine its benign and malignant, early detection of clinical, early treatment of malignant tumors provide a reliable basis for diagnosis. Traditional cytology rely on light microscopy to observe the morphology of the nucleus, sometimes due to interference of background components, or atypical morphology, susceptible to the pathologist’s subjective factors. We use DNA ploidy analysis and immunohistochemical staining in the diagnosis of difficult cases, hoping to contribute to the diagnosis of difficult cytology. 1 Materials and Methods 1.1 Materials Selection Chengdu Military Region General Hospital 2013-09-2014-12 Difficulties