论文部分内容阅读
作者用四季海棠(Begonia semperflorens Link et Otto)单核晚期花粉的花药进行培养,并获得了花粉植株。试验表明,诱导愈伤组织频率较高的培养基为B_5+激动素1毫克/升+2,4-D2毫克/升+蔗糖5％;诱导愈伤组织分化苗较好的培养基为MS+玉米素10毫克/升+吲哚乙酸0.5毫克/升+蔗糖 3％(但玉米素浓度要逐步提高)。将分化形成的无根苗扦插在蛭石基质上,浇入一定量加有0.1毫克/升IAA的MS减半培养液,两周后即可生根。分化的植株经根尖染色体检查2n=x=18,证明为单倍体花粉植株。
The authors used monolayer Begonia semperflorens Link et Otto anther culture of mononuclear pollen and obtained pollen plants. The results showed that the medium with higher callus induction rate was B5 + 1 mg / L + 2,4-D2 mg / L + 5% sucrose. The best medium for inducing callus differentiation was MS + zeatin 10 mg / L + indole acetic acid 0.5 mg / L + sucrose 3% (but the zein concentration should be gradually increased). The rooted seedlings formed by differentiation were cut into vermiculite substrate and poured into a certain amount of MS medium supplemented with 0.1 mg / L IAA for two weeks before rooting. Differentiated plants by root tip chromosome examination 2n = x = 18, proved to be haploid pollen plants.