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实验结合营养学和毒理学的实验方法,对饲料中不同剂量半胱胺盐酸盐预混剂(Cysteamine Hydrochloride,CSH,含量27%)对大口黑鲈(Micropterus salmoides)生长性能、血液生理生化指标、消化酶活性和器官组织学的影响进行了评估,研究其对大口黑鲈生长性能的影响,并对CSH作为大口黑鲈饲料添加剂的安全性进行评价。结果表明:CSH添加量为648 mg/kg组的鱼体特定生长率和摄食率以及肠道胃蛋白酶、胰蛋白酶活性均显著高于对照组(P<0.05),但饲料系数、存活率、肥满度、内脏比和肝指数、血液生理生化指标、淀粉酶活性对照组相比差异不显著(P>0.05),该组大口黑鲈肝脏和肠道显示轻微损伤。添加32400 mg/kg组,各项生长指标、存活率、胃蛋白酶和胰蛋白酶活性均显著小于对照组(P<0.05)。根据大口黑鲈生长、消化酶活性及组织切片结果确定受试物(含CSH27%)对大口黑鲈56d最大未观察到有害作用剂量(NOAEL)为24 mg/kg.w.d,折算CSH对大口黑鲈56dNOAEL为6.5 mg/kg.w.d。
Experiments were conducted to study the effects of dietary Cysteamine Hydrochloride (CSH) content of 27% on the growth performance, blood physiological and biochemical parameters of Micropterus salmoides in combination with nutritional and toxicological experimental methods. , Digestive enzyme activity and organ histology were evaluated to investigate their effects on growth performance of largemouth bass and the safety of CSH as a feed additive for largemouth bass was evaluated. The results showed that the specific growth rate, food intake rate, intestinal pepsin and trypsin activity of CSH at 648 mg / kg were significantly higher than those at control (P <0.05), but the feed efficiency, Full scale, visceral ratio and liver index, blood physiological and biochemical indicators, amylase activity in the control group compared to no significant difference (P> 0.05), the group of largemouth bass liver and intestine show slight damage. Add 32400 mg / kg group, the growth index, survival rate, pepsin and trypsin activity were significantly less than the control group (P <0.05). According to the growth, digestive enzyme activity and histological section results of the largemouth bass, the maximum NOAEL of the test substance (including CSH27%) on the 56th day was 24 mg / kg.wd, and the conversion of CSH to large black The perch 56dNOAEL was 6.5 mg / kg.wd.