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目的探讨Y-box结合蛋白1(YB-1)与增殖细胞核抗原(PCNA)在胃癌中的表达及其相关性。方法采用免疫组化法检测120例胃癌患者癌组织及20例患者癌旁组织中YB-1、PCNA蛋白的表达,分析YB-1和PCNA表达与胃癌患者临床病理特征的关系。结果 YB-1和PCNA在胃癌组织中的阳性表达率高于癌旁组织(70.8%vs.20.0%和60.8%vs.30.0%)(P<0.05);两者的表达水平均与胃癌患者的肿瘤浸润深度、细胞分化程度、TNM分期及淋巴结转移密切相关(P<0.05)。YB-1阳性表达组5年生存率低于阴性表达组(17.6%vs.34.3%)(P<0.05)。胃癌组织中YB-1与PCNA表达呈正相关(r=0.311,P<0.01)。结论 YB-1和PCNA在胃癌组织中高表达,并与临床病理学特征密切相关。联合检测胃癌组织YB-1和PCNA表达有助于胃癌的诊断以及评估其恶性程度和胃癌患者的预后。
Objective To investigate the expression and correlation of Y-box binding protein 1 (YB-1) and proliferating cell nuclear antigen (PCNA) in gastric cancer. Methods The expression of YB-1 and PCNA protein in 120 cases of gastric cancer tissues and 20 cases of paracancerous tissues was detected by immunohistochemistry. The relationship between the expression of YB-1 and PCNA and clinicopathological features of gastric cancer was analyzed. Results The positive rates of YB-1 and PCNA in gastric cancer tissues were higher than those in adjacent tissues (70.8% vs. 20.0% and 60.8% vs. 30.0%, P <0.05). The expression levels of both YB- The depth of tumor invasion, cell differentiation, TNM stage and lymph node metastasis were closely related (P <0.05). The 5-year survival rate of YB-1 positive expression group was lower than that of negative expression group (17.6% vs.34.3%) (P <0.05). There was a positive correlation between YB-1 and PCNA expression in gastric cancer (r = 0.311, P <0.01). Conclusion YB-1 and PCNA are highly expressed in gastric cancer and are closely related to clinicopathological features. Combined detection of gastric cancer YB-1 and PCNA expression contributes to the diagnosis of gastric cancer and to assess its malignancy and prognosis of patients with gastric cancer.