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目的:探讨真核细胞起始因子eIF-4A在正常及不同级别病变宫颈组织中的表达及其临床意义。方法:选取青岛大学医学院附属医院妇科2011-01-01-2012-06-01行手术治疗或活检确诊的120例患者,其中低度鳞状上皮内病变组织(CINⅠ级)30例,高度鳞状上皮内病变组织(CINⅡ和Ⅲ级)30例及宫颈癌组织60例(51例鳞癌,9例非鳞癌)。另选取同期手术切除的30例正常宫颈组织作为对照。应用免疫组织化学方法测定不同宫颈组织中eIF-4A蛋白的表达水平,探讨其与宫颈癌临床病理参数之间的关系。结果:eIF-4A蛋白的阳性表达率在正常宫颈组织(0)、低度鳞状上皮内病变(46.7%)、高度鳞状上皮内病变(76.7%)及宫颈鳞状细胞癌组织(78.4%)中逐渐增高,差异有统计学意义,χ2=54.421,P<0.001;且趋势检验有统计学意义,χ2=47.111,P<0.001;但是高度鳞状上皮内病变组织和宫颈鳞状细胞癌组织中eIF-4A的阳性表达差异无统计学意义,χ2=0.034,P=0.854。eIF-4A蛋白表达与宫颈癌组织学类型无关,χ2=0.592,P=0.442;与宫颈癌的病理分级有关,χ2=6.161,P=0.046;与临床分期有关,χ2=7.515,P=0.006。结论:eIF-4A可能参与了宫颈病变发生和发展的过程,可能在宫颈病变的初始过程即发挥了作用。
Objective: To investigate the eukaryotic initiation factor eIF-4A in normal and different grades of cervical lesions and its clinical significance. Methods: A total of 120 patients underwent surgical treatment or biopsy in the department of gynecology, Affiliated Hospital of Medical College of Qingdao University from January 2011 to January 01, 2012. Thirty patients with low grade squamous intraepithelial lesion (CINⅠ grade) Like intraepithelial lesion tissue (CIN Ⅱ and Ⅲ grade) 30 cases and cervical cancer tissue 60 cases (51 cases of squamous cell carcinoma, 9 cases of non-squamous cell carcinoma). Another 30 cases of normal cervical tissue resection simultaneously selected as a control. The expression of eIF-4A protein in different cervical tissues was detected by immunohistochemistry and the relationship between the expression of eIF-4A protein and the clinicopathological parameters of cervical cancer was analyzed. Results: The positive expression rates of eIF-4A protein in normal cervical tissue (0), low grade squamous intraepithelial lesion (46.7%), highly squamous intraepithelial lesion (76.7%) and cervical squamous cell carcinoma tissue (78.4% ), The difference was statistically significant, χ2 = 54.421, P <0.001; and the trend test was statistically significant, χ2 = 47.111, P <0.001; but a high degree of squamous intraepithelial lesion and cervical squamous cell carcinoma There was no significant difference in the positive expression of eIF-4A, χ2 = 0.034, P = 0.854. χ2 = 0.592, P = 0.442). The expression of eIF-4A was related to the pathological grade of cervical cancer (χ2 = 6.161, P = 0.046). The correlation between eIF-4A and clinical stage was χ2 = 7.515, P = 0.006. Conclusions: eIF-4A may be involved in the development of cervical lesions and may play an important role in the initial process of cervical lesions.