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用芒果多酚处理人宫颈癌细胞(Hela)后,采用MTT法检测细胞的存活率;Hoechst 33258荧光染色和流式细胞术检测细胞凋亡、细胞周期分布,同时用Western blot检测p53,p21,c-myc,cyclin D1蛋白质水平变化.结果显示:0.025,0.05,0.1,0.2,0.4 mg/mL的芒果多酚溶液能明显抑制Hela细胞的增殖,并呈剂量和时间依赖性;荧光染色后,大多数细胞内可以看见浓密的荧光颗粒,并出现核收缩;流式细胞术分析发现,与未处理组比较,芒果多酚作用后的G1期Hela细胞数显著增多,G2期细胞数逐渐减少;凋亡率明显高于对照组;Western blot检测显示芒果多酚上调p53,p21蛋白水平,下调c-myc,cyclinD1蛋白水平.以上结果表明芒果多酚能明显抑制人宫颈癌Hela细胞的增殖,诱导其凋亡.
After treatment of human cervical cancer cells (Hela) with mango polyphenols, the cell viability was detected by MTT assay. The apoptosis and cell cycle distribution were detected by Hoechst 33258 staining and flow cytometry. The expressions of p53, p21, c-myc, cyclin D1 protein levels.The results showed that: 0.025,0.05,0.1,0.2,0.4 mg / mL of mango polyphenol solution can significantly inhibit the proliferation of Hela cells in a dose-and time-dependent manner; fluorescence staining, In most cells, dense fluorescent particles were observed and nuclear contraction appeared. Flow cytometry analysis showed that compared with untreated group, the number of Hela cells in G1 phase and the number in G2 phase decreased gradually after mango polyphenols treatment. Western blot showed that the protein levels of p53 and p21 were up-regulated and the protein levels of c-myc and cyclinD1 were down-regulated in mango polyphenols.The results showed that mango polyphenols could significantly inhibit the proliferation and induction of human cervical cancer Hela cells Its apoptosis.