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目的了解武汉地区婴幼儿腹泻轮状病毒感染特点及型别分布。方法分别应用酶联免疫吸附试验法(ELISA)和聚丙烯酰胺凝胶电泳技术(PAGE),对2000年9月至2003年9月在武汉市儿童医院就诊的3625例婴幼儿腹泻患儿粪便进行轮状病毒抗原检测和轮状病毒基因组RNA检测及分型。结果共检出阳性标本2810例(阳性率77.5%),其中ELISA阳性检测率为78.5%PAGE阳性检测率为58.1%,2种方法均检出阳性率为43.8%;A组轮状病毒电泳型有2个亚组,第1亚组为短型S,第2亚组为长型L1、L2、L3、L4,本次调查以长型L1多见,占97.8%,短型只占2.2%。结论武汉地区婴幼儿腹泻50%以上由轮状病毒引起,2岁以下的婴幼儿为易感人群;发病季节以每年10月至次年元月为高峰期;流行病学特点与各省、市报道一致;临床症状呕吐、代谢性酸中毒、咳嗽等以及显微镜检查脂肪球阳性,均与轮状病毒感染密切相关(P<0.005)。
Objective To understand the characteristics and distribution of infantile diarrhea rotavirus infection in Wuhan. Methods Enzyme linked immunosorbent assay (ELISA) and polyacrylamide gel electrophoresis (PAGE) were performed on 3625 infantile diarrhea infants in Wuhan Children’s Hospital from September 2000 to September 2003 Rotavirus antigen detection and rotavirus genomic RNA detection and typing. Results A total of 2810 positive samples (positive rate 77.5%) were detected. The positive detection rate of ELISA was 78.5%, the positive detection rate was 58.1%, and the positive rate of both methods was 43.8%. A group of rotavirus electrophoresis type There are 2 subgroups, the first subgroup is short S, the second subgroup is long L1, L2, L3, L4, the survey is more common with long L1, accounting for 97.8%, short 2.2% . Conclusions More than 50% of infant diarrhea in Wuhan is caused by rotavirus, and infants under 2 years of age are susceptible. The incidence season is peaked from October to January of the following year. Epidemiological characteristics are reported in all provinces and cities The clinical symptoms of vomiting, metabolic acidosis, cough, and microscopic examination of fat globules were positive and rotavirus infection were closely related (P <0.005).