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本实验共用雄性猕猴(Macaca mula-ila)12只,分成三组:(1)对照组;(2)X射线200仑一次急性照射后第9天取出一侧睾丸,作为一次急性照射组;(3)把200仑总剂量分成10份,每隔24小时照射20仑,最后一次照射后的第9天取出一侧睾丸,作为分次累积照射组。一次照射组和分次照射组中A型精原细胞和B型精原细胞数量都明显低于对照组。一次照射情况下,该两类精原细胞各占对照的65.43%和33.09%;分次照射时各占对照的5.57%和0%;畸变细胞百分率在分次照射组中要比一次照射组高2.3倍。一次照射和分次照射后精母细胞数量都下降,分别占对照组的84.27%和6.92%,但只有分次照射组与对照组的差异是显著的(P<0.001)。畸变精母细胞在分次照射组中高达50.21%,并分布在67.5%的精细管切面内;一次照射后,畸变精母细胞较少,只有0.78%。随着精细管切面直径的收缩,支持细胞数量有所增加,尤以分次照射组为甚,在统计学上有显著意义。上述结果表明,分次累积照射对生殖细胞的损伤效应比一次急性照射严重,可能是射线对生殖细胞直接致损和抑制细胞分裂两者综合作用的结果,至于两类精原细胞的受损程度和数量消减的程度都不一致,可能是辐射敏感性差异的反映。
In this experiment, 12 male Macaca mula-ila were divided into three groups: (1) control group; (2) one side of testis was removed on the 9th day after acute radiation of X-ray; 3) The total dose of 200 Lun was divided into 10 portions, and irradiated every 20 hours for 24 hours. On the 9th day after the last irradiation, one side of the testis was taken out as a fractionated cumulative irradiation group. The number of type A spermatogonia and type B spermatogonia in one irradiation group and fractionated irradiation group were significantly lower than those in the control group. In one irradiation, the two types of spermatogonia accounted for 65.43% and 33.09% of the control respectively; each fractional irradiation accounted for 5.57% and 0% of the control respectively; and the percentage of aberrant cells in the fractionated irradiation group was higher than that in the first irradiation group 2.3 times. The number of spermatocytes decreased after one irradiation and fractionated irradiation, accounting for 84.27% and 6.92% of the control group respectively, but only the difference between the fractionated irradiation group and the control group was significant (P <0.001). The aberrant spermatocytes were up to 50.21% in fractionated irradiation group and distributed in 67.5% of the fine tube section. After one irradiation, the number of abnormal spermatocytes was only 0.78%. With the narrowing of the diameter of the fine tube, the number of supporting cells increased, especially in the fractionated irradiation group, which was statistically significant. The above results show that fractionated cumulative irradiation on germ cell damage than a serious acute radiation may be the direct effects of radiation on germ cell damage and inhibition of cell division results of both the combined effect of the two types of spermatogonial damage And the degree of abatement are all inconsistent, which may be a reflection of the difference of radiation sensitivity.