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目的:优选三脉紫菀总黄酮的提取、纯化工艺条件。方法:采用正交试验设计,以总黄酮含量为指标,优选三脉紫菀总黄酮的提取工艺条件;以总黄酮静态吸附量和解吸率为考察指标筛选大孔吸附树脂型号,并优化其纯化总黄酮的工艺条件。结果:最佳提取工艺条件为乙醇体积分数60%,浸提温度50℃,浸提3次,料液比1∶10。HPD-500型大孔吸附树脂对三脉紫菀总黄酮有较好的吸附分离性能,以16 mg总黄酮/g树脂的上柱量配制成50 g.L-1的上柱液,上柱后静置1 h,用4 BV水洗除杂,用2 BV 70%乙醇洗脱,收集洗脱液。此工艺条件下,总黄酮纯度76.7%,树脂富集倍数3.83。结论:该优选工艺简便、实用,可为三脉紫菀的工业生产提供参考。
Objective: To optimize the extraction and purification of total flavonoids from Aster pulmonaria. Methods: The orthogonal experimental design was adopted. The total flavonoids content was taken as the index to optimize the extraction conditions of total flavonoids from Aster, and the quantity of macroporous adsorption resin was screened based on the static adsorption capacity and desorption rate of total flavonoids. Flavonoids process conditions. Results: The optimal extraction conditions were ethanol volume fraction 60%, extraction temperature 50 ℃, leaching three times, the ratio of solid to liquid 1:10. HPD-500 macroporous resin has good adsorption and separation performance on the total flavonoids of Aster pulmonaria, with the amount of 16 mg total flavonoids / g resin on the preparation of 50 gL-1 on the column liquid, on the column after standing 1 h, washed with 4 BV washed with 2 BV 70% ethanol, the eluent was collected. Under this process conditions, the purity of total flavonoids was 76.7% and the resin enrichment factor was 3.83. Conclusion: The optimal process is simple and practical, which can provide reference for the industrial production of Aster pulmonaria.