日本藤泽公司通过对他克莫司产生菌筑波链霉菌的选育，发酵条件优化，提取方法改良，以及建立简单、快速、可信度高的ＨＰＬＣ定量分析方法，顺利实现他克莫司的工业化生产，产率比原始菌株和原始发酵条件提高了３００倍，产物的异构化得到控制，产品纯度达到９９％以上。 Japan’s Fujisawa company through the selection of tacrolimus bacteria Streptomyces, Optimization of fermentation conditions, extraction methods to improve, and the establishment of simple, fast, high confidence in the HPLC quantitative analysis of the smooth realization of tacrolimus Industrial production, the yield was 300 times higher than the original strain and the original fermentation conditions, the isomerization of the product was controlled, the purity of the product reached more than 99%.