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小麦苗期性状能够指示品种的耐盐性。本研究以小麦骨干亲本燕大1817与品系北农6号衍生的230个重组自交系为材料,利用2013年3个不同时间的水培试验数据和已经构建的SSR和SNP高密度遗传连锁图谱分别对正常和盐胁迫条件下根数和最长根长等7个苗期性状进行QTL定位。利用完备复合区间作图法(ICIM)共检测到69个加性效应QTL(LOD≥2.5),分布于除1A染色体外的所有20条染色体上,单个QTL解释的表型变异率为2.70%~19.00%。有46个QTL的增效效应来自于燕大1817,有23个QTL的增效效应来自于北农6号。有12个QTL能够在3个或3个以上的环境中被检测到,在燕大1817中定位到稳定的多分蘖主效QTL QTn.cau-7BS.1和盐胁迫条件下特异表达的根数QTL QRn.cau-2A,解析了小麦骨干亲本燕大1817的繁茂性和抗逆性遗传基础,为解析小麦品种耐盐遗传机制和耐盐性的分子标记辅助选择提供了重要信息。
Wheat seedling traits can indicate the salt tolerance of varieties. In this study, 230 recombinant inbred lines derived from the backbone parent YanDa 1817 and BeiNong 6 were used as experimental materials. Based on the data from three hydroponic experiments in 2013 and the high-density genetic linkage map of SSR and SNP QTL mapping was conducted on seven seedling traits, including root number and longest root length, under normal and salt stress respectively. A total of 69 additive-effect QTLs (LOD≥2.5) were detected by complete composite interval mapping (ICIM) and distributed on all 20 chromosomes except chromosome 1A. The phenotypic variation explained by a single QTL was 2.70% 19.00%. The synergistic effect of 46 QTLs came from YanDa 1817. The synergistic effect of 23 QTLs came from Beinong 6. Twelve QTLs were detected in three or more environments. In YanDa 1817, QTn.cau-7BS.1, a major QTL for multiple tillering, and the number of specific expression under salt stress QTL QRn.cau-2A, and analyzed the genetic basis of lushness and stress resistance of the backbone parent YanDa 1817 in wheat, which provided important information for the analysis of molecular marker-assisted selection of salt tolerance mechanism and salt tolerance in wheat cultivars.