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目的在体外培养条件下,探讨阿魏酸钠(SF)拮抗氧化低密度脂蛋白(Ox-LDL)的作用机制。方法在体外培养人脐静脉内皮细胞(HUVEC)中分别和同时加入SF和Ox-LDL,采用基因芯片技术分析内皮细胞中白细胞介素-1β(IL-1β)的基因表达改变;用实时PCR技术(RT-PCR)检测IL-1βmRNA表达,用酶联免疫吸附试验(ELISA)检测IL-1β蛋白表达。结果基因芯片检测结果显示,HUVEC受Ox-LDL诱导后,IL-1β基因表达上调,比空白对照组高2.4倍;而采用SF+Ox-LDL处理后,IL-1β基因表达下调,是Ox-LDL组的2.6倍。RT-PCR与ELISA结果显示,Ox-LDL组与空白对照组比较,IL-1βmRNA及蛋白表达均明显增高;SF+Ox-LDL组与空白对照组比较,IL-1βmRNA表达差异无统计学意义;蛋白表达则明显降低。结论结论 Ox-LDL可诱导内皮细胞的IL-1β表达明显增强,阿魏酸钠能够抑制Ox-LDL活性,下调IL-1β的mRNA表达,可减少IL-1β对血管内皮细胞的损害。
Objective To investigate the mechanism of sodium ferulate (SF) antagonizing oxidized low-density lipoprotein (Ox-LDL) in vitro. Methods SF and Ox-LDL were added into human umbilical vein endothelial cells (HUVECs) in vitro and separately. The gene expression of interleukin-1β (IL-1β) in endothelial cells was analyzed by gene chip technique. The expression of IL-1βmRNA was detected by RT-PCR and the expression of IL-1βprotein was detected by enzyme linked immunosorbent assay (ELISA). Results The results of microarray assay showed that the expression of IL-1β was up-regulated in HUVECs induced by Ox-LDL, which was 2.4-fold higher than that of the blank control group. However, the expression of IL- LDL group 2.6 times. The results of RT-PCR and ELISA showed that the expression of IL-1β mRNA and protein in Ox-LDL group was significantly higher than that in blank control group. There was no significant difference of IL-1β mRNA expression between SF + Ox-LDL group and blank control group. Protein expression was significantly reduced. Conclusions Ox-LDL can induce the expression of IL-1β in endothelial cells significantly. Sodium ferulate can inhibit the activity of Ox-LDL and down-regulate the mRNA expression of IL-1β, which can reduce the damage of vascular endothelial cells induced by IL-1β.