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从全国十多个省市送检的疑似患多发浆膜炎与关节炎猪的病料中分离到 32株细菌,进行了细菌形态观察、培养特性和生化特性鉴定;根据副猪嗜血杆菌 16SrRNA序列设计引物进行PCR扩增, 将 822bp扩增片段连入T 载体后测序, 再与GenBank(M75065 )中的序列进行比对,表明其与国外副猪嗜血杆菌菌株 16SrRNA序列的同源性为 97%以上,确定为副猪嗜血杆菌(Haemophilusparasuis, Hps)。将其中的 15株按Kieletein Rapp Gabriedson(KRG)琼脂扩散血清分型方法进行血清型鉴定,结果为血清 5型 3株、血清 4型 4株、血清 13型2株、血清 12型 2株,另外有 4株不能进行分型。该结果表明副猪嗜血杆菌在我国广泛存在。
Thirty-two bacteria were isolated from the suspected cases of multiple serositis and arthritis pigs in more than ten provinces and cities in China. The bacterial morphology, culture characteristics and biochemical characteristics were identified. According to H. parasuis 16S rRNA Sequence design of primers for PCR amplification, the 822bp amplified fragment was ligated into the T vector and sequenced, and then compared with the sequence in GenBank (M75065), indicating that its homology with foreign Haemophilus parasuis strain 16SrRNA sequence 97% or more, identified as Haemophilus parasuis (Hps). Fifteen of them were serotyped by Kieletein Rapp-Gabriedson (KRG) agar diffusion-serotyping. The results showed that there were 3 serotypes 5, 4 serotypes 4, 2 serotypes 13 and 2 serotypes 12, 4 strains can not be typed. The results show that H. parasuis exists widely in our country.