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目的探究徐州地区结核分枝杆菌的基因型分布特征及其与利福平耐药基因突变形成的关系。方法选取徐州市传染病医院结核病实验室基因型毒株500株,其中含有经罗氏比例法鉴定的耐利福平临床分离株300株和利福平敏感200株,采用间隔区寡核苷酸分型(spoligotyping)分析其基因型结构及其与利福平抗性基因的突变关系。结果徐州地区的结核分枝杆菌的基因分型主要为北京家族基因群(61.2%)和非北京家族基因群(38.8%),利福平耐药和敏感组北京家族基因群的构成比差异无统计学意义(2=0.09,P>0.05);北京家族531位点、516位点以及531和526位点突变率分别为55.49%、10.99%和7.69%与非北京家族33.90%、21.19%和15.25%相比,差异有统计学意义(P<0.05)。结论徐州地区结核分枝杆菌基因型为北京家族基因群和非北京家族基因群,非北京家族基因群具有基因多态性,两者与利福平耐药率无明显相关,两者在单531、单516以及531和526利福平耐药位点突变率存在差异。
Objective To explore the genotype distribution of Mycobacterium tuberculosis in Xuzhou and its relationship with the mutation of rifampicin resistance gene. Methods A total of 500 genotype strains of tuberculosis laboratory in Xuzhou Infectious Disease Hospital were selected, including 300 Rifampicin clinical isolates and 200 Rifampicin susceptible strains identified by Roche proportion method. The spacer oligonucleotide (Spoligotyping) analysis of its genotype structure and rifampicin resistance gene mutations. Results The genotypes of Mycobacterium tuberculosis in Xuzhou area were mainly from Beijing family genes (61.2%) and non-Beijing family genes (38.8%), and rifampicin resistant and susceptible groups The mutation rates of Beijing family members 531, 516 and 531 and 526 were 55.49%, 10.99% and 7.69% respectively, which were 33.90% and 21.19% of those in non-Beijing family (2 = 0.09, P> 0.05) Compared with 15.25%, the difference was statistically significant (P <0.05). Conclusion The genotypes of Mycobacterium tuberculosis in Xuzhou area are Beijing family gene family and non-Beijing family gene family. The non-Beijing family gene family has gene polymorphism. There is no significant correlation between the two genotypes and the rifampin resistance rate. , Single 516 and 531 and 526 rifampicin resistance sites mutation rate differences.