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目的:探讨苯并(a)芘[B(a)P]代谢产物反式二氢二醇环氧苯并芘[anti-7,8,-dihydrodiol-9,10-epoxidebenzo(a)pyrene,BPDE]引起的细胞FHIT基因突变情况。方法:PCR、RT-PCR、多聚酶链聚合反应-单链构象多态性分析(PCR-SSCP)。结果:PCR-SSCP结果提示反式BPDE诱发的恶性转化细胞及其裸鼠成瘤细胞FHIT基因的Exon1-9出现了异常的小片断。结论:反式BPDE可作用于细胞染色体3p14.2的脆性部位FRA3B,引起FHIT基因的重排或缺失,导致FHIT基因功能失活。推测FHIT基因可能是反式BPDE致癌作用的靶分子之一。
Objective: To explore the metabolites of benzo(a)pyrene[B(a)P] trans-dihydrodiol epoxybenzopyrene[anti-7,8,-dihydrodiol-9,10-epoxidebenzo(a)pyrene, BPDE ] Caused mutations in the cellular FHIT gene. Methods: PCR, RT-PCR, polymerase chain reaction-single strand conformation polymorphism analysis (PCR-SSCP). RESULTS: PCR-SSCP results showed abnormal small fragments of Exon1-9 in the FHIT gene induced by trans BPDE in malignant transformed cells and tumorigenic nude mice. CONCLUSION: Trans BPDE can act on FRA3B, a fragile site on chromosome 3p14.2, resulting in rearrangement or deletion of FHIT gene, resulting in inactivation of FHIT gene. It is speculated that the FHIT gene may be one of the target molecules for trans-BPDE carcinogenesis.