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目的采用液质联用技术检测金锁固精丸中非法添加的枸橼酸西地那非。方法选用C18色谱柱(2.1mm×100mm,5μm),以乙腈-0.01mol·L-1乙酸铵溶液梯度洗脱,对此制剂的提取液进行液质联用分析,通过与对照品的色谱和质谱行为比较,检测此制剂中枸橼酸西地那非成分。结果在该金锁固精丸中检出枸橼酸西地那非。结论本方法简便、灵敏、准确,专属性强,可分析检测此制剂中的枸橼酸西地那非。
Objective To determine the illegal addition of sildenafil citrate in Jin Suo Gu Jing Wan by liquid chromatography and mass spectrometry. Methods C18 column (2.1mm×100mm, 5μm) was used to elute the acetonitrile-0.01mol·L-1 ammonium acetate solution. The extract of this preparation was analyzed by liquid chromatography and mass spectrometry. Comparison of the mass spectrometry behaviors and detection of sildenafil citrate in this formulation. As a result, sildenafil citrate was detected in the gold lock solid pill. Conclusion This method is simple, sensitive, accurate, and highly specific. It can be used for the analysis and detection of sildenafil citrate in this preparation.