目的获得７型腺病毒疫苗株ＤＮＡ左侧０～１７５ｍｕ片段克隆，分析０～４８ｍｕ片段（末端倒置重复序列、包装信号位点和Ｅｌａ区）核苷酸序列。方法从Ａｄ７疫苗株感染的Ａ５４９细胞提取Ａｄ７ＤＮＡ，将其０３～１７５ｍｕ片段克隆到质粒ｐＡｄ７Ｔ，并用自动和银染方法测序。结果获得了Ａｄ７疫苗株左侧１７５ｍｕ片段克隆，测定了左侧１７３７ｂｐ核苷酸序列，其中Ｅｌａ区所编码的６３００、２４０００、２８０００等３个蛋白的ＤＮＡ序列，与Ｇｏｍｅｎ株的同源性分别为９８９％、９７３％和９７５％，推导编码氨基酸的同源性分别为９６６％、９６５％和９６９％。这３个蛋白与Ｇｒｉｄｅｒ株的同源性分别为１００％、９９７％、和９９７％；推导编码氨基酸的同源性分别为１００％、９９１％和９９２％。结论Ａｄ７疫苗株左侧１７３８ｂｐ核苷酸与Ａｄ７Ｇｏｍｅｎ株和Ｇｒｉｄｅｒ株相应片段，具有高度的同源性。 OBJECTIVE: To obtain the 0 ~ 175 mu fragment on the left side of the DNA of type 7 adenovirus vaccine and to analyze the nucleotide sequence of 0 ~ 48 mu fragment (terminal inverted repeat, packaging signal site and Ela region). Methods Ad7 DNA was extracted from A549 cells infected with Ad7 vaccine strain, and the 03 ~ 175 mu fragment was cloned into plasmid pAd7T and sequenced automatically by silver staining. Results A 175mu fragment was cloned on the left side of the Ad7 vaccine strain. The 1737bp nucleotide sequence of the left side of the Ad7 vaccine strain was obtained. The DNA sequence of the 6300, 24000, 28000 and so on encoded by the Ela region was homologous with that of the Gomen strain 989%, 973% and 975%, respectively. The deduced amino acids homologies were 966%, 965% and 969%, respectively. The homologies of these three proteins to Grider strains were 100%, 99.7%, and 99.7%, respectively. The deduced amino acid identities were 100%, 991% and 992%, respectively. Conclusion The 1738bp nucleotide on the left of Ad7 vaccine strain has high homology with the corresponding fragments of Ad7Gomen strain and Grider strain.