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为了在金膜固相载体上固定肌红蛋白单克隆抗体(MbAb),通过在金膜上生长一层巯基酸和巯基醇的混合自组装分子膜(SAMs),用原子力显微镜(AFM)和X射线光电子能谱仪(XPS)分析样品的性质,再以1-(3-二甲基氨基丙基)3-乙基碳化二亚胺盐酸(EDC.HCl)作为催化剂,自组装膜和抗体的氨基发生偶联反应,将抗体固定在金膜表面,并进行肌红蛋白抗原(MbAg)的测定。结果显示,通过条件优化实验,发现50 mmol/L的巯基16酸和巯基11醇混合乙醇溶液,60℃处理金膜3 h后,再偶联40 mg/L的MbAb 3 h,固定效果较好,对MbAg可测至30μg/L。该方法为利用磁电阻生物传感器诊断心肌梗塞提供了理论和实验依据。
In order to immobilize the myoglobin monoclonal antibody (MbAb) on the gold membrane solid phase carrier, a series of mixed self-assembled molecular films (SAMs) of thiol and mercapto alcohol were grown on gold film, The properties of the sample were analyzed by X-ray photoelectron spectroscopy (XPS), and the membrane and antibody were self-assembled using 1- (3- dimethylaminopropyl) 3-ethylcarbodiimide hydrochloride (EDC.HCl) Amino coupling reaction, the antibody is immobilized on the surface of the gold membrane, and myoglobin antigen (MbAg) determination. The results showed that 50 mmol / L mercapto-16 acid and mercapto-11 alcohol mixed ethanol solution were found to be the best immobilization conditions after 3 h treatment at 60 ℃ for 3 h and then 40 mg / L MbAb for 3 h , MbAg can be measured to 30μg / L. The method provides a theoretical and experimental basis for the diagnosis of myocardial infarction with a magnetoresistive biosensor.