论文部分内容阅读
目的探讨免疫抑制剂雷帕霉素对肝癌细胞生物学行为的影响。方法建立肝癌裸鼠原位移植瘤模型80只,随机分为空白对照组、环孢霉素(CsA)处理组、雷帕霉素常规剂量处理组和高剂量处理组,每组20只。用药2周后观察肿瘤生长情况,RT-PCR方法检测肿瘤组织中CD44v6 mRNA的表达。结果雷帕霉素常规剂量组及高剂量组肿瘤体积较空白对照组明显缩小,CD44v6的表达显著下调(P<0.05);CsA组肿瘤体积较之空白对照组明显增大,CD44v6的表达显著上调(P<0.05)。结论CsA可促进肝癌的生长和转移;雷帕霉素具有显著抑制肝癌侵袭转移的作用,能抑制肝癌细胞CD44v6基因的表达。
Objective To investigate the effect of rapamycin, an immunosuppressive agent, on the biological behavior of hepatoma cells. Methods Eighty orthotopic transplantation tumor models of hepatocellular carcinoma in nude mice were established and randomly divided into blank control group, cyclosporine (CsA) group, conventional rapamycin group and high dose group. Tumor growth was observed 2 weeks after treatment and the expression of CD44v6 mRNA was detected by RT-PCR. Results Compared with the control group, the tumor volume of the rapamycin group and the high-dose rapamycin group were significantly reduced, the expression of CD44v6 was significantly decreased (P <0.05); the tumor volume of CsA group was significantly increased compared with the blank control group, and the CD44v6 expression was significantly increased (P <0.05). Conclusion CsA can promote the growth and metastasis of hepatocellular carcinoma. Rapamycin can significantly inhibit the invasion and metastasis of hepatocellular carcinoma and inhibit the expression of CD44v6 gene.