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本文的主要目的是研究大鼠原代成骨细胞在细胞外基质蛋白微图形上的生长行为。为此,采用微接触印刷术在聚苯乙烯材料表面制备了一系列具有不同形状和不同尺度的纤连蛋白微图形,激光共聚焦扫描显微镜观察结果表明,微图形形态规整,微图形在细胞培养过程中较为稳定。细胞实验表明,细胞优先黏附在蛋白区域,在不同的微图形表面表现出不同的细胞形态与铺展行为,蛋白微图形对细胞的黏附、铺展、排列以及细胞生长方向等行为具有非常明显的影响。因此,通过在材料表面构建细胞外基质蛋白微图形对生物材料特别是骨替代材料进行表面改性,可以有效地调控细胞的生长行为,有利于进一步研究材料与成骨细胞相互作用的界面生物学行为,为发展具有能够自主调控成骨细胞生长行为的骨替代植入材料提供实验依据。
The main purpose of this paper is to investigate the growth behavior of rat primary osteoblasts on micro-patterns of extracellular matrix proteins. For this reason, a series of microfibrils with different shapes and different scales were prepared on the surface of polystyrene by micro-contact printing. The results of laser scanning confocal microscope showed that the morphology of micro-pattern was regular and the micro- The process is more stable. Cell experiments showed that cells preferentially adhered to the protein region, showing different cell morphology and spreading behavior on different micro-patterned surfaces. The protein micro-pattern had a very significant effect on cell adhesion, spreading, arrangement and cell growth direction. Therefore, surface modification of biomaterials, especially bone substitute materials, by constructing micromatrixes of extracellular matrix proteins on the surface of materials can effectively regulate the growth behavior of cells and facilitate the further study of interfacial biology of the interaction of materials with osteoblasts Behavior, provide an experimental basis for the development of bone substitute implants capable of autonomously regulating the growth of osteoblasts.