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目的检测手机微波辐照2 h后人眼晶状体上皮细胞(human lens epithelial cells,LECs)的DNA损伤及其修复情况。方法LECs分为辐照组和假辐照组,置于sXc-1800细胞辐照(发射217 Hz脉冲调制的1．8 GHz微波)系统内连续波辐照2 h,辐照强度比吸收率(specific absorption rate,SAR)分别为0、1、2、3、4W/kg,辐照后0、30、60、120、240min分别进行彗星试验检测尾长和尾相,以判定细胞DNA的损伤及其修复情况。结果1和2 W/kg辐照诱导的DNA损伤与假辐照组相比,在各个检测时段的差异均无统计学意义(P＞0．05)；辐照后即刻进行的检测发现,3和4W/kg组DNA损伤与假辐照组相比,差异有统计学意义(P＜0．01),3 W/kg组的差异在修复30 min后仍然存在,修复60 min后消失,而4 W/kg组的差异在各检测时段持续存在。结论SARa≤3 W/kg的1．8 GHz手机微波辐照2 h对体外培养的人眼晶状体上皮细胞不产生或产生可修复DNA损伤,4 W/kg的辐照剂量可导致细胞DNA不可逆性损伤。
Objective To detect DNA damage and repair of human lens epithelial cells (LECs) after 2 h of cell phone microwave irradiation. Methods LECs were divided into irradiation group and sham irradiation group. The irradiated LECs were exposed to sXc-1800 (irradiation of 217 GHz pulsed 1.8 GHz microwave) for 2 h. The specific absorption ( Specific absorption rate (SAR) were 0, 1, 2, 3 and 4 W / kg respectively. The tail length and tail phase were detected by comet assay at 0, 30, 60, 120 and 240 min after irradiation to determine the DNA damage Its repair situation. Results Compared with the sham irradiation group, the DNA damage induced by 1 and 2 W / kg irradiation had no significant difference at each detection time (P> 0.05). The detection immediately after irradiation showed that 3 (P <0.01). The differences of 3 W / kg group still existed after 30 min of repair and disappeared after 60 min of repair, while the difference of DNA damage in 4 W / kg group was not significant Differences in the 4 W / kg group persisted throughout the test period. CONCLUSION: Radiation of 1.8 W cell phone with SARa ≤ 3 W / kg for 2 h did not produce or produce repairable DNA damage in cultured human lens epithelial cells in vitro. Radiation dose of 4 W / kg resulted in irreversible cellular DNA damage.