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目的调查类鼻疽伯克霍尔德氏菌在广西地区的详细分布情况。方法于2007年底对广西地区的14个地级市进行了大规模调查,共采集了77个地点的154个土壤样品和130个水样。采样通过细菌分离培养方法、16S rDNA基因测序和鞭毛基因检测的方法进行鉴定分析,对类鼻疽伯克霍尔德氏菌检出情况的地理分布进行绘图记录。结果 154份土壤样品中分离纯化出13株类鼻疽伯克霍尔德氏菌,阳性率为8.4%,没有在水样中发现该菌;分离纯化结果与16S rDNA基因测序和鞭毛基因检测的结果完全一致。分离纯化类鼻疽伯克霍尔德氏菌阳性的采样点分布在中东、南宁、钦州、放城、北海5个地区。结论广西地区的类鼻疽伯克霍尔德氏菌主要分布在北回归线以南,南海附近地区。
Objective To investigate the detailed distribution of Burkholderia pseudomallein in Guangxi. Methods By the end of 2007, a large-scale survey was conducted on 14 prefecture-level cities in Guangxi. A total of 154 soil samples and 130 water samples from 77 sites were collected. The samples were identified and analyzed by bacterial isolation and culture method, 16S rDNA gene sequencing and flagella gene detection, and the geographical distribution of Burkholderia pseudomallei was plotted and recorded. Results 13 strains of Burkholderia pseudomallei were isolated and purified from 154 soil samples. The positive rate was 8.4%. The bacteria were not found in water sample. The results of isolation and purification and 16S rDNA gene sequencing and flagella gene analysis were complete Consistent. Burkholderia isolates positive samples collected in the distribution of the Middle East, Nanning, Qinzhou, Liberation City, Beihai five regions. Conclusions Burkholderia pseudomallei in Guangxi is mainly distributed in the area south of the Tropic of Cancer and near the South China Sea.