Objective: To systematically evaluate and compare the effects of electro-acupuncture combined with biofeedback training and simple biofeedback training on the intervention of patients with functional defecation disorder.Methods: Computer retrieval was performed to search randomized controlled trials about electroacupuncture combined with biofeedback training in the treatment of patients with functional defecation disoder from January 2000 to January 2019 via websites, including CNKI, Wanfang data knowledge service platform, CBM, VIP, PubMed, EMBase, Cochrane Library, Web of Science, and Springerlink, JBI evidence-based Nursing database, RNAO, Nursing Consult, BIOSIS, Medline and so on. Screening was performed according to the inclusion and exclusion criteria, data were extracted, literature quality was evaluated, and Meta analysis was performed on the extracted data using RevMan5.3 software. Results: Five studies including 363 subjects were included. The results of meta-analysis showed that the effective rate of electroacupuncture combined with biofeedback group (combined group) in the treatment of functional defecation disorder was higher than that of biofeedback group (control group) [RR=1.29, 95％CI (1.17, 1.42),P<0.00001], the difficulty score of defecation in combined group was lower than that of control group [MD=–0.71, 95％ CI (–1.22, –0.21),P=0.006], and anal rest pressure was lower than that of control group [MD=–0.44, 95％ CI (-0.80, –0.09),P=0.02]. Tubing maximum systolic pressure is lower than the control group [MD=–2.06, 95％ CI (–3.60, –0.52),P=0.009).Conclusion: Acupuncture combined with biofeedback can effectively improve the defecation difficulty of patients with functional defecation disorder, as well as the anal resting pressure and the maximum anal systolic pressure. Due to the limitation of the number of included literatures and the heterogeneity among evaluation indexes, the evaluation of defecation difficulty score and anal dynamics still needs to be further evaluated under the support of clinical studies with more centers, high quality and large sample size.