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稀土离子能否诱导海洋生物的金属硫蛋白(MT)合成,将影响到MT对海水重金属的指示作用。以菲律宾蛤仔(Ruditapes philippinarum)为试验动物,采用含镧(Ⅲ)离子(La3+)的人工海水对其进行暴露培养,测定得到蛤仔鳃部和内脏中MT含量随暴露浓度和时间的变化。结果表明,La3+能够促进蛤仔MT的诱导,当以1×102μg.L-1La3+对蛤仔暴露培养5 d时,鳃部和内脏MT含量均达到最高水平,分别为0.135±0.014μmol-SH/g和0.276±0.025μmol-SH/g;1×10-1~1×102μg.L-1的La3+对鳃部MT的诱导作用较大,继续增加La3+浓度,诱导作用有所降低;1×10-1~1×104μg.L-1的La3+对内脏MT均有较好诱导,并基本保持恒定,表明La3+对机体MT的诱导具有一定限度。值得注意的是,我国某些海域的海水稀土离子浓度已达到可诱导双壳类MT合成的水平。
Whether rare earth ions can induce the synthesis of metallothionein (MT) in marine organisms will affect the indication of MT on heavy metals in seawater. Ruditapes philippinarum was used as experimental animal, and exposed to artificial seawater containing lanthanum (Ⅲ) ion (La3 +). The content of MT in the gill and viscera of the clams was determined with the exposure concentration and time. The results showed that La3 + could promote the induction of MT of Ruditapes longitticum. The contents of MT in gill and viscera reached the highest levels when exposed to 1 × 102μg.L-1La3 + for 5 days, respectively, which were 0.135 ± 0.014μmol-SH / g and 0.276 ± 0.025μmol-SH / g, respectively. La3 + of 1 × 10-1 ~ 1 × 102μg.L-1 had a greater effect on the gill MT induction and continued to increase the concentration of La3 + -1 ~ 1 × 104μg.L-1 of La3 + on the visceral MT were better induced, and remained basically constant, indicating that La3 + on the body’s induction of MT has a certain limit. It is worth noting that the seawater rare earth ion concentration in some areas of China has reached the level that can induce bivalve MT synthesis.