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曾经报道不用保护剂或用甘油或二甲基亚砜作保护剂冰冻保存恶性疟原虫红内期。本文报道用含7.5%甘油的Alsevers溶液低温保存恶性疟原虫和间日疟原虫红内期的结果。这种新的保护剂含有9份Alsevers液和1份甘油。以1.5ml分装于一种特制的小管内,高压消毒后,贮存5℃备用。冰冻时每管加入0.5ml抗凝含虫猴血,混匀,先置于含酒精的干冰中速冻,而后将小管贮存于-70℃冷冻箱,以供体内研究用。复苏时,用自来水冲洗解冻。用这种方法冰冻保存5株恶性疟原虫和2株间日疟原虫红内期均获成功。其中恶性疟原虫越南Oak Knoll株和间日疟原虫溪桑
It has been reported that cryopreserved Plasmodium falciparum internodes without preservatives or with glycerol or dimethylsulfoxide as a preservative. This paper reports the results of cryopreservation of Plasmodium falciparum and Plasmodium vivax in an Alsevers solution containing 7.5% glycerol. This new preservative contains 9 parts Alsevers solution and 1 part glycerol. 1.5ml sub-packed in a special small tube, autoclaved, stored at 5 ℃ standby. Add 0.5ml anticoagulant entomological monkey blood to each tube while freezing, mix well and place on a quick freeze in alcohol-containing dry ice before storing the tube in a -70 ° C freezer for in-vivo studies. During recovery, rinse with tap water to thaw. Cryopreservation of 5 P. falciparums and 2 P. vivax strains by this method were successful. Among them, Plasmodium falciparum Vietnam Oak Knoll strain and Plasmodium vivax